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Broad-spectrum electrochemical immunosensor based on one-step electrodeposition of AuNP-Abs and Prussian blue nanocomposite for organophosphorus pesticide detection.

School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, 255049, China. Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, 255049, China. Zibo City Key Laboratory of Agricultural Product Safety Traceability, Zibo, China. School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, 255049, China. gym@sdut.edu.cn. Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, 255049, China. gym@sdut.edu.cn. Zibo City Key Laboratory of Agricultural Product Safety Traceability, Zibo, China. gym@sdut.edu.cn.

Bioprocess and biosystems engineering. 2021;(3):585-594
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Abstract

Broad-spectrum antibodies can effectively recognize substances with similar structures and have broad application prospects in field rapid detection. In this study, broad-spectrum antibodies (Abs) against organophosphorus pesticides (OPs) were used as sensitive recognition elements, which could effectively recognize most OPs. Gold nanoparticles (AuNPs) have good biocompatibility. It combined with Abs to form a gold-labeled probe (AuNPs-Abs), which enhances the effective binding of antibodies to nanomaterials. Prussian blue (PB) was added to electrodeposition solution to enhance the conductivity, resulting in superior electrochemical performance. The AuNP-Abs-PB composite film was prepared by electrodeposition on the electrode surface to improve the anti-interference ability and stability of the immunosensor. Under the optimal experimental conditions, the immunosensor had a wide detection range (IC20-IC80: 1.82 × 10-3-3.29 × 104 ng/mL) and high sensitivity. Most importantly, it was simple to be prepared and could be used to detect multiple OPs.